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KMID : 0624620120450030153
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BMB Reports
2012 Volume.45 No. 3 p.153 ~ p.158
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Geft is dispensable for the development of the second heart field
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Fan Xiongwei
Hou Ning
Fan Kaiji
Yuan Jiajia
Mo Xiaoyang
Deng Yun
Wan Yongqi
Teng Yan
Yang Xiao
Wu Xiushan
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Abstract
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Geft is a guanine nucleotide exchange factor, which can specifically activate Rho family of small GTPase by catalyzing the exchange of bound GDP for GTP. Geft is highly expressed in the excitable tissue as heart and skeletal muscle and plays important roles in many cellular processes, such as cell proliferation, migration, and cell fate decision. However, the in vivo role of Geft remains unknown. Here, we generated a Geft conditional knockout mouse by flanking exons 5-17 of Geft with loxP sites. Cre-mediated deletion of the Geft gene in heart using Mef2c-Cre transgenic mice resulted in a dramatic decrease of Geft expression. Geft knockout mice develop normally and exhibit no discernable phenotype, suggesting Geft is dispensable for the development of the second heart field in mouse. The Geft conditional knockout mouse will be a valuable genetic tool for uncovering the in vivo roles of Geft during development and in adult homeostasis.
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KEYWORD
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Conditional null allele, Cre-loxP, FLPe-Frt, Geft, Heart development
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